Journal article
Ex vivo culture of chimeric antigen receptor T cells generates functional CD8 T cells with effector and central memory-like phenotype
P Neeson, A Shin, KM Tainton, P Guru, HM Prince, SJ Harrison, S Peinert, MJ Smyth, JA Trapani, MH Kershaw, PK Darcy, DS Ritchie
Gene Therapy | NATURE PUBLISHING GROUP | Published : 2010
DOI: 10.1038/gt.2010.59
Abstract
The anti-tumor efficacy of adoptively transferred T cells requires their in vivo persistence and memory polarization. It is unknown if human chimeric antigen receptor (CAR)-expressing T cells can also undergo memory polarization. We examined the functional status of CAR CD8 T cells, re-directed to Lewis Y antigen (LeY-T), throughout a period of ex vivo expansion. Immediately before culture CD8 T cells comprised a mixture of phenotypes including naive (CD45RA /CCR7 /CD27 /CD28 /perforin), central memory (CM, CD45RA /CCR7 lo /CD27 /CD28 /perforin lo), effector memory (EM, CD45RA /CCR7 /CD27 /CD28 /perforin mod) and effector (Eff, CD45RA /CCR7 /CD27 /CD28 /perforin hi) cells. After transduction..
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Awarded by Leukemia and Lymphoma Society
Funding Acknowledgements
Funding for this study came from the National Health and Medical Research Council of Australia, Program Grant no. 454569, the Peter MacCallum Cancer Center Morris Family Grant and the Leukemia Lymphoma Society (6073-06). MH Kershaw and PK Darcy were supported by a NHMRC Senior Research Fellowship and Career Development Award respectively. MJ Smyth and JA Trapani were supported by NHMRC Senior Principal Research Fellowships.